Oligonucleotides containing new fluorescent 1-phenylethynylpyrene and 9,10-bis(phenylethynyl)anthracene uridine-2′-carbamates: synthesis and properties

The synthesis of two novel fluorescent uridine-2′-carbamate phosphoramidites is described. The reagents carrying fluorescent polyaromatic hydrocarbons 1-phenylethynylpyrene (PEPy) or 9,10-bis(phenylethynyl)anthracene (BPEA) are suitable for oligonucleotide synthesis. Prepared oligonucleotide conjugates show strong dye emissions at 401 and 485 nm, but low FRET rate when located in the oligonucleotide duplex. The dyes show considerable compensation of the usual carbamate duplex destabilization. The possible explanation of both effects is binding of PEPy and BPEA to the minor groove of the DNA duplex.     

Hetero-calix[4]pyrroles: incorporation of furans, thiophenes, thiazoles or fluorenes as a part of the macrocycle

Furans, thiazoles, fluorene or thiophene incorporated calix[4]pyrrole analogues were synthesized and characterized. The synthesis was achieved by utilization of various building blocks such as 7, 13, 14, 18 and 21. Acid catalyzed condensation of those building blocks with acetone or meso-disubstituted dipyrromethanes afforded desired macrocycles.     

Electrochemical Preparation of Poly(Malachite Green) Film Modified Nafion‐Coated Glassy Carbon Electrode and Its Electrocatalytic Behavior Towards NADH,Dopamine and Ascorbic Acid

Poly(malachite green) film modified Nafion‐coated glassy carbon electrodes have been prepared by potentiodynamic cycling in malachite green solution. The pH of polymerisation solution has only minor effect on film formation. Electrochemical quartz crystal microbalance (EQCM) was used to monitor the growth of the poly(malachite green) film. Cyclic voltammogram of the poly(malachite green) film shows a redox couple with well‐defined peaks. The redox response of the modified electrode was found to be depending on the pH of the contacting solution. The peak potentials were shifted to a less positive region with increasing pH and the dependence of the peak potential was found to be 56 mV per pH unit. The electrocatalytic behavior of poly(malachite green) film modified Nafion‐coated glassy carbon electrodes was tested towards oxidation of NADH, dopamine, and ascorbic acid. The oxidation of dopamine and ascorbic acid occurred at less positive potential on poly(malachite green) film compared to bare glassy carbon electrode. In the case of NADH, the overpotential was reduced substantially on modified electrode. Finally, the feasibility of utilizing poly(malachite green) film electrode in analytical estimation of ascorbic acid was demonstrated in flow injection analysis.     

聚吡咯水杨酸修饰电极的电化学行为及水杨酸含量测定

本用电化学方法制备了聚吡咯－水杨酸修饰电极；并进行了电化学行为的研究，经电化学处理该是电极对水杨酸根具有选择性响应，响应线性范围在１．０×１０＾－＾５－１．０×１０＾－＾３ｍｏｌ／Ｌ之间。电极斜率为４０ｍＶ／ｐＣ，该电极具有较好的稳定性，响应时间在１８０－３００ｓ之间；电极寿命可达６０ｄ。     

异咯嗪蒙脱石修饰电极的电化学行为

应用十六烷基三甲基溴化铵对蒙脱石进行改性，成功地制备稳定性良好的异咯嗪蒙脱石修饰电采，用循环伏安法对此修饰电极的电化学行为进行了研究。测定了异咯嗪在蒙脱石膜内的化学扩散系数，对异咯嗪在改性蒙脱石中电极反应机制进行了探讨。     

三正辛胺修饰电极伏安测定痕量金的研究

用化学修饰电极伏安测定痕量金,Kalcher K等曾采用阴离子交换剂碳糊修饰电极及打萨宗碳糊修饰电极检测100～300μg/L的金。本文采用三正辛胺(TOA)修饰玻碳电极,在1.5 mol/L介质中,Au(Ⅲ)在+0.16 V(vs SCE)处有一灵敏的不可逆还原峰。检出限为0.1μg/L。灵敏度比文献方法高千倍。Au(Ⅲ)浓度在5×10~(-7)～5×10~(-9)mol/L范围内峰高与浓度     

纳米银自组装电极的制备及对高氯酸二茂铁的电化学响应

采用自组装的方法制备了纳米银粒子修饰金电极,并运用循环伏安法、交流阻抗谱探讨了该电极的电化学特性.研究了高氯酸二茂铁在该修饰电极上的直接电化学行为.实验结果表明,高氯酸二茂铁在该修饰电极上具有良好的电流响应.用示差脉冲法测定高氯酸二茂铁,其氧化峰电流与浓度在4.0×10-6～5.0×10-4 mol/L范围呈良好线性关系,线性方程为:Ip(μA)=0.0236c(μmol/L)-0.0975,线性相关系数为0.9982,检出限为2.3×10-7 mol/L(信噪比为3).     

N—（3—二茂铁乙酰胺基）丙基吡咯聚合物修饰的葡萄糖电极的研究

于铂电极上修饰一层Ｎ－（３－二茂铁乙酰胺基）丙基吡咯聚合物膜，应用循环伏安法对聚合物的电化学性能进行研究，发现了Ｎ－位取代吡咯聚合物的电活性大大降低，聚合物中 二茂铁基团氧化还原性能稳定，制成酶电极后，在＋０．２Ｖ以葡萄糖有一明显的催化峰，而对抗坏血酸，尿酸则几乎没有呼应，该葡萄糖电极性能稳定，连续工作十天，响应值基本不变。     

壳聚糖修饰玻碳电极卷积伏安法测定环境水中的EDTA

制备了壳聚糖修饰玻碳电极 ,研究了Fe(EDTA) -在修饰电极上的吸附还原行为 ,用卷积伏安法通过Fe(EDTA) -的检测测定了环境水中的EDTA。在优化的实验条件下 ,峰电流值与 8.0× 1 0 -7～ 5 .0× 1 0 -6mol L的EDTA呈线性关系 ,回归方程为epp′ =0 .75 2 5c - 0 .661 3,r =0 .991 ,最低检出限为 5 .0× 1 0 -7mol L ,5次测定的相对标准偏差小于 5 .8%。对实际样品测定的回收率为98 1 %～ 1 0 5 % ,对比HPLC的结果 ,相对偏差小于 5 %。     

THE SYNTHESIS OF MODIFIED DIPHENYL OXIDE RESIN

1. INTRODUCTION Poly (diphenyl oxide) (DPO) resins are a series of heat-resisting thermosetting resins. In 1960s, the West House Company applied them to make H class insulting materials and got an extensive use successfully [1]. But due to the three main…